In vivo labeling and turnover of prolyl hydroxylase and a related immunoreactive protein
Date of Original Version
Prolyl hydroxylase and an immunologically related protein (CRP) were purified from neonatal rabbit skin at various time periods following administration of 3H-leucine. The peak incorporation of label into prolyl hydroxylase was found to be 12 hours, while peak incorporation into CRP occurred within 2 hours. Semi-log plots of the loss of radioactivity from these protein pools against time indicated an apparent T 1 2 for prolyl hydroxylase of 78 hours, and a T 1 2 of CRP of 44 hours. Calculated Kd values indicate that that breakdown of active enzyme does not account for the amount of CRP found in tissues. © 1976.
Biochemical and Biophysical Research Communications
Chichester, Clinton O., George C. Fuller, and George J. Cardinale. "In vivo labeling and turnover of prolyl hydroxylase and a related immunoreactive protein." Biochemical and Biophysical Research Communications 73, 4 (1976): 1056-1062. doi:10.1016/0006-291X(76)90230-8.