Document Type
Article
Date of Original Version
3-1-2023
Abstract
Premise: To genetically discriminate subspecies of the common reed (Phragmites australis), we developed real-time quantitative (qPCR) assays for identifying P. australis subsp. americanus, P. australis subsp. australis, and P. australis subsp. berlandieri.
Methods and Results: Utilizing study-generated chloroplast DNA sequences, we developed three novel qPCR assays. Assays were verified on individuals of each subspecies and against two non-target species, Arundo donax and Phalaris arundinacea. One assay amplifies only P. australis subsp. americanus, one amplifies P. australis subsp. australis and/or P. australis subsp. berlandieri, and one amplifies P. australis subsp. americanus and/or P. australis subsp. australis. This protocol enhances currently available rapid identification methods by providing genetic discrimination of all three subspecies.
Conclusions: The newly developed assays were validated using P. australis samples from across the United States. Application of these assays outside of this geographic range should be preceded by additional testing.
Publication Title, e.g., Journal
Applications in Plant Sciences
Volume
11
Issue
2
Citation/Publisher Attribution
Lindsay, D. L., Guan, X., Harms, N. E., Cronin, J. T., Meyerson, L. A., & Lance, R. F. (2023). DNA assays for genetic discrimination of three Phragmites australis subspecies in the United States. Applications in Plant Science, 11(2), e11512. https://doi.org/10.1002/aps3.11512
Available at: https://doi.org/10.1002/aps3.11512
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.