Season, anthocyanin supplementation, and flight training have mixed effects on the antioxidant system of migratory European Starlings
Document Type
Article
Date of Original Version
7-1-2021
Abstract
Migratory birds engage in 2 periods of endurance flight annually as they travel between summer breeding and overwintering grounds, and such endurance flights likely incur oxidative costs. These costs may differ between fall and spring migration, especially for females who must prepare for breeding and egg laying in spring. The objective of this study of a migratory bird was to test proposed hypotheses about how key components of the female's antioxidant system differ in response to flight training in the fall and spring and to dietary antioxidant supplementation. We hand raised female European Starlings (Sturnus vulgaris) and fed them either a diet supplemented with dietary anthocyanins or a diet without added anthocyanins. We flew females in a wind tunnel for 15 days during fall and spring migration seasons and measured over time oxidative lipid damage (d-ROMs) and 3 components of the antioxidant system: nonenzymatic antioxidant capacity (OXY), uric acid, and glutathione peroxidase (GPx) activity. Prior to flight training, OXY and oxidative damage were lower in females during spring compared with fall, and females fed a low-antioxidant diet had consistently higher circulating uric acid. GPx activity decreased more in spring immediately after a long-duration flight. Females fed a high-antioxidant diet had a greater decrease in OXY after the 15-day flight training. Flight-trained females had higher circulating uric acid than untrained females immediately after the longest-duration flight and decreased GPx activity after the 15-day flight training. In sum, females upregulated enzymatic and nonenzymatic endogenous antioxidants in spring, and females fed a diet with less antioxidants appear to compensate by increasing circulating uric acid. Our findings emphasize the important role of dietary antioxidants for birds during migration, and similar flights in fall and spring likely represent distinct oxidative challenges in the life history of female birds. LAY SUMMARY Migratory birds incur oxidative costs during their long endurance flights, and these costs may differ between fall and spring migration, especially for females who must prepare for breeding and egg laying in spring. We fed female European Starlings diets with different quantities of anthocyanin, a dietary antioxidant, and then measured during both fall and spring migration periods how their antioxidant system responded to diet and two weeks of daily flight in a wind tunnel. Females upregulated some key components of their endogenous antioxidant system in spring and had lower oxidative damage than females in fall, and females fed less dietary antioxidants appear to have compensated by increasing circulating uric acid. Similar flights in fall and spring likely represent distinct oxidative challenges for female songbirds, and dietary antioxidants play an important role in modulating the antioxidant system.
Publication Title, e.g., Journal
Ornithology
Volume
138
Issue
3
Citation/Publisher Attribution
Frawley, Abigail E., Kristen J. DeMoranville, Katherine M. Carbeck, Lisa Trost, Amadeusz Bryla, Maciej Dzialo, Edyta T. Sadowska, Ulf Bauchinger, Barbara J. Pierce, and Scott R. McWilliams. "Season, anthocyanin supplementation, and flight training have mixed effects on the antioxidant system of migratory European Starlings." Ornithology 138, 3 (2021). doi: 10.1093/ornithology/ukab023.