Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins
Date of Original Version
Tetraspore mucilage and rhizoids of Champia parvula (C. Agardh) Harvey were characterized with histochemical stains and fluorescein isothiocyanate (FITC)-labelled lectins and examined by SEM and light microscopy. The extracellular mucilage of attached tetraspores, rhizoids, and holdfasts stained positive for sulphated and carboxylated polysaccharides. Using a variety of FITC-lectins to probe the mucilage of attached tetraspores, we detected the sugar haptens α-D-mannose, α-D-glucose, β-D-galactose, and N-acetylgalactosamine, N-acetylglucosamine and α-L-fucose were not detected with FITC-lectins specific for these sugar haptens. Spores, rhizoids, and holdfast cells stained for protein, whereas their accompanying mucilage did not stain for protein.
Publication Title, e.g., Journal
Apple, Martha E., Marilyn M. Harlin, and Joanna H. Norris. "Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins." Phycologia 35, 3 (1996): 245-252. doi: 10.2216/i0031-8884-35-3-245.1.