Title
Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins
Document Type
Article
Date of Original Version
1-1-1996
Abstract
Tetraspore mucilage and rhizoids of Champia parvula (C. Agardh) Harvey were characterized with histochemical stains and fluorescein isothiocyanate (FITC)-labelled lectins and examined by SEM and light microscopy. The extracellular mucilage of attached tetraspores, rhizoids, and holdfasts stained positive for sulphated and carboxylated polysaccharides. Using a variety of FITC-lectins to probe the mucilage of attached tetraspores, we detected the sugar haptens α-D-mannose, α-D-glucose, β-D-galactose, and N-acetylgalactosamine, N-acetylglucosamine and α-L-fucose were not detected with FITC-lectins specific for these sugar haptens. Spores, rhizoids, and holdfast cells stained for protein, whereas their accompanying mucilage did not stain for protein.
Publication Title
Phycologia
Volume
35
Issue
3
Citation/Publisher Attribution
Apple, Martha E., Marilyn M. Harlin, and Joanna H. Norris. "Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins." Phycologia 35, 3 (1996): 245-252. doi:10.2216/i0031-8884-35-3-245.1.