Title

Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins

Document Type

Article

Date of Original Version

1-1-1996

Abstract

Tetraspore mucilage and rhizoids of Champia parvula (C. Agardh) Harvey were characterized with histochemical stains and fluorescein isothiocyanate (FITC)-labelled lectins and examined by SEM and light microscopy. The extracellular mucilage of attached tetraspores, rhizoids, and holdfasts stained positive for sulphated and carboxylated polysaccharides. Using a variety of FITC-lectins to probe the mucilage of attached tetraspores, we detected the sugar haptens α-D-mannose, α-D-glucose, β-D-galactose, and N-acetylgalactosamine, N-acetylglucosamine and α-L-fucose were not detected with FITC-lectins specific for these sugar haptens. Spores, rhizoids, and holdfast cells stained for protein, whereas their accompanying mucilage did not stain for protein.

Publication Title

Phycologia

Volume

35

Issue

3

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