Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins

Document Type

Article

Date of Original Version

1-1-1996

Abstract

Tetraspore mucilage and rhizoids of Champia parvula (C. Agardh) Harvey were characterized with histochemical stains and fluorescein isothiocyanate (FITC)-labelled lectins and examined by SEM and light microscopy. The extracellular mucilage of attached tetraspores, rhizoids, and holdfasts stained positive for sulphated and carboxylated polysaccharides. Using a variety of FITC-lectins to probe the mucilage of attached tetraspores, we detected the sugar haptens α-D-mannose, α-D-glucose, β-D-galactose, and N-acetylgalactosamine, N-acetylglucosamine and α-L-fucose were not detected with FITC-lectins specific for these sugar haptens. Spores, rhizoids, and holdfast cells stained for protein, whereas their accompanying mucilage did not stain for protein.

Publication Title, e.g., Journal

Phycologia

Volume

35

Issue

3

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