Isolation of the Virulence Gene(s) in the New, Live, Oral Vaccine Candidate Salmonella typhimurium SR-11 Fad

Author

James H. Allen, University of Rhode Island

Date of Award

1998

Degree Type

Thesis

Degree Name

Master of Science in Microbiology

Department

Biochemistry, Microbiology, and Molecular Genetics

First Advisor

Paul S. Cohen

Abstract

A DNA fragment containing the defective virulence gene(s) in the live oral vaccine candidate Salmonella typhimurium SR-11 Fad was isolated. Genomic DNA fragments from the vaccine candidate, constructed by Tn10d::cam transposon mutagenesis, were probed with the 1.4 Kbp chloramphenicol resistance gene contained in Tn10d::cam and analyzed by Southern hybridization. Two of the fragments, an 8.0 Kbp Hind III fragment and a 4.5 Kbp Pst I fragment, were isolated, cloned into E. coli HB 101, and mapped. The Pst I fragment, containing approximately 2.9 Kbp of flanking genomic DNA, was subcloned into the sequencing vector pBluescript II SK + (Stratagene, La Jolla, CA) and mapped.

Recommended Citation

Allen, James H., "Isolation of the Virulence Gene(s) in the New, Live, Oral Vaccine Candidate Salmonella typhimurium SR-11 Fad" (1998). Open Access Master's Theses. Paper 1097.
https://digitalcommons.uri.edu/theses/1097