Single Wall Carbon Nanotubes for the Detection of Misfolded Proteins in Neuro-degenerative Disorders
Major
Chemical Engineering
Second Major
French
Advisor
Nadeem, Aceer
Advisor Department
Chemical Engineering
Date
4-2024
Abstract
Single wall carbon nanotubes (SWCNT) are one-dimensional carbon nanomaterials made up of a singular layer of graphene. These nanotubes can be functionalized with different molecules for various bio-sensing/imaging applications. SWCNTs are unique in their ability to be used as long-term multiplexed sensors due to their photostability and ability to remain fluorescent.
This project focused on the functionalization of SWCNTs to detect misfolded proteins for early detection of neuro-degenerative diseases like Alzheimers. Currently, there is no simple way to detect these misfolded proteins and diagnose for diseases like Alzheimers, Parkinsons, or ALS, and a confirmed diagnosis can take upwards of six months. This project aimed to identify a functionalized SWCNT for the detection of Amyloid - β42, the protein that will be targeted for Alzheimers. Five different functionalized SWCNTs were made and compared in both protein-free and protein-saturated conditions. The functionalized SWCNTs exposed to Amyloid - β42 were then examined in two complex medias. This was done to imitate non-ideal environments and see how the fluorescence and biosensing capabilities of the SWCNTs change. Experimental findings indicate a preferred functionalized SWCNT for the detection of Amyloid - β42. Future studies can expand the types of functionalizations and examine how more complex medias made to mimic intracellular conditions would affect the biosensing capabilities of the functionalized SWCNTs.