Ixodes scapularis: Salivary kininase activity is a metallo dipeptidyl carboxypeptidase

Document Type

Article

Date of Original Version

1-1-1998

Abstract

Saliva and salivary gland homogenates of Ixodes scapularis contain a dipeptidyl carboxypeptidase activity that accounts for the previously described salivary kininase activity of this tick. Reversed phase HPLC and laser desorption mass spectrography of the reaction products identified bradykinin fragment 1-7 and 1-5 as being produced subsequent to incubation of purified salivary kininase with bradykinin. The activity was inhibited by captopril and EDTA and was activated by cobalt and manganese, a behavior similar to that displayed by angiotensin-converting enzymes of vertebrate and invertebrate origins.

Publication Title, e.g., Journal

Experimental Parasitology

Volume

89

Issue

2

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