Date of Award
Doctor of Philosophy in Physics
Acidity is considered an alternative biomarker for targeting diseased tissues, such as in the acute phase of inflammation or tumors with poor prognosis. The family of pH Low Insertion Peptides (pHLIPs) are known markers of tissue acidity which provide a useful method for the diagnosis and/or treatment of disease by extracellular or intracellular delivery of imaging agents or therapeutic molecules. In my research, I employ fluorescence and CD spectroscopic techniques to understand how pHLIP's pH-dependent interactions with and insertion into the lipid bilayer of the cell membrane are affected by its sequence and conjugation with cargo and how this correlates with the targeting of acidic cells in cell culture and in mouse models.
Visca, Hannah, "BIOPHYSICAL CHARACTERIZATION OF PHLIP AND PHLIP-CARGO CONJUGATES DEVELOPED FOR IMAGING AND THERAPY" (2023). Open Access Dissertations. Paper 1556.