Microfluidic inverse phase ELISA via manipulation of magnetic beads
Document Type
Article
Date of Original Version
3-1-2011
Abstract
We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are preencapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (μIPELISA). μIPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use μIPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng μl-1 of digoxigenin- labeled amplicon, μIPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct μIPELISA in two parallel microchannels. © Springer-Verlag 2010.
Publication Title, e.g., Journal
Microfluidics and Nanofluidics
Volume
10
Issue
3
Citation/Publisher Attribution
Chen, Hong, Assem Abolmatty, and Mohammad Faghri. "Microfluidic inverse phase ELISA via manipulation of magnetic beads." Microfluidics and Nanofluidics 10, 3 (2011): 593-605. doi: 10.1007/s10404-010-0692-2.