Microfluidic inverse phase ELISA via manipulation of magnetic beads

Authors

Hong Chen, University of Rhode Island
Assem Abolmatty, University of Rhode Island
Mohammad Faghri, University of Rhode Island

Document Type

Article

Date of Original Version

3-1-2011

Abstract

We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid reagents to detect an antigenic analyte. These isolated but accessible plugs are preencapsulated in a microchannel by capillary force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (μIPELISA). μIPELISA has distinctive advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying, thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use μIPELISA to detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng μl-1 of digoxigenin- labeled amplicon, μIPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct μIPELISA in two parallel microchannels. © Springer-Verlag 2010.

Publication Title, e.g., Journal

Microfluidics and Nanofluidics

Volume

10

Issue

3

Citation/Publisher Attribution

Chen, Hong, Assem Abolmatty, and Mohammad Faghri. "Microfluidic inverse phase ELISA via manipulation of magnetic beads." Microfluidics and Nanofluidics 10, 3 (2011): 593-605. doi: 10.1007/s10404-010-0692-2.