Induction by β-estradiol of vitellogenin in striped bass (Morone saxatilis): Characterization and quantification in plasma and mucus
Document Type
Article
Date of Original Version
1-1-1992
Abstract
Striped bass (Morone saxatilis) were implanted with R-estradiol to induce the production of vitellogenin, the egg yolk precursor produced by the liver. Electrophoretic analysis revealed that β-estradiol caused marked production of a plasma protein of apparent molecular mass 170 kDa. Size exclusion chromatography suggested that the estradiol-induced protein circulated as a dimer. This protein was purified from the plasma of estradiol-treated fish by DEAE-agarose column chromatography and used to induce antibodies in rabbits and goats. Western blots revealed that the antiserum bound to the putative vitellogenin in plasma from estradiol-treated fish and adult females, but not with any proteins in male plasma. Western blot of ovarian extract revealed several smaller immunoreactive protein bands and supported the identity of the purified protein as vitellogenin. A competitive ELISA was developed with sensitivity in a range from 8 to 1000 ng/ml. Plasma concentrations of adult females during their spawning migration ranged from 100 to 600 μg/ml Western blot of mucus extract revealed the presence of a 170-kDa protein in vitellogenic female fish along with several minor bands ranging from 50 to 110 kDa. Positive immunoreactivity was present in the surface mucus of all females and in none of the males collected during a spawning migration in the Hudson liver. © 1992.
Publication Title, e.g., Journal
General and Comparative Endocrinology
Volume
88
Issue
1
Citation/Publisher Attribution
Kishida, Mitsuyo, Thomas R. Anderson, and Jennifer L. Specker. "Induction by β-estradiol of vitellogenin in striped bass (Morone saxatilis): Characterization and quantification in plasma and mucus." General and Comparative Endocrinology 88, 1 (1992). doi: 10.1016/0016-6480(92)90191-L.