Nitrogen isotope ratio of cyanobacterial chlorophyll: Chemostat vs. batch culture
Date of Original Version
Nitrogen isotope records of chloropigments can be used to reconstruct photic zone processes. However, the 15N offset between chlorophyll and biomass (ε por=δ 15N biomass-δ 15N chlorophyll) of cyanobacteria and eukaryotes grown in laboratory batch cultures differs significantly and the cause of the difference remains unknown. Here, in four experiments with Synechocystis sp. PCC 6803, values of ε por were statistically invariant when cultures were maintained at pH 6.5-9.5 under constant growth conditions in a chemostat. The results suggest that the negative values of ε por observed for freshwater cyanobacteria are not artifacts of batch culturing. © 2012 Elsevier Ltd.
Publication Title, e.g., Journal
Tsao, Leah E., Rebecca S. Robinson, Meytal B. Higgins, and Ann Pearson. "Nitrogen isotope ratio of cyanobacterial chlorophyll: Chemostat vs. batch culture." Organic Geochemistry 49, (2012). doi: 10.1016/j.orggeochem.2012.06.001.