Preparation and biocompatibility of chitosan microcarriers as biomaterial

Authors

Xi Guang Chen, Ocean University of China
Cheng Sheng Liu, Ocean University of China
Chen Guang Liu, Ocean University of China
Xiang Hong Meng, Ocean University of China
Chong M. Lee, University of Rhode Island
Hyun Jin Park, Korea University

Document Type

Article

Date of Original Version

1-1-2006

Abstract

Chitosan microcarriers (100-200 μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120°C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial. © 2005 Elsevier B.V. All rights reserved.

Publication Title, e.g., Journal

Biochemical Engineering Journal

Volume

27

Issue

3

Citation/Publisher Attribution

Chen, Xi Guang, Cheng Sheng Liu, Chen Guang Liu, Xiang Hong Meng, Chong M. Lee, and Hyun Jin Park. "Preparation and biocompatibility of chitosan microcarriers as biomaterial." Biochemical Engineering Journal 27, 3 (2006). doi: 10.1016/j.bej.2005.08.021.