Document Type
Article
Date of Original Version
1-2016
Abstract
In many biomedical contexts ranging from chemotherapy to tissue engineering, it is beneficial to sequentially present bioactive payloads. Explicit control over the timing and dose of these presentations is highly desirable. Here, we present a capsule-based delivery system capable of rapidly releasing multiple payloads in response to ultrasonic signals. In vitro, these alginate capsules exhibited excellent payload retention for up to 1 week when unstimulated and delivered their entire payloads when ultrasonically stimulated for 10–100 s. Shorter exposures (10 s) were required to trigger delivery from capsules embedded in hydrogels placed in a tissue model and did not result in tissue heating or death of encapsulated cells. Different types of capsules were tuned to rupture in response to different ultrasonic stimuli, thus permitting the sequential, on-demand delivery of nanoparticle payloads. As a proof of concept, gold nanoparticles were decorated with bone morphogenetic protein-2 to demonstrate the potential bioactivity of nanoparticle payloads. These nanoparticles were not cytotoxic and induced an osteogenic response in mouse mesenchymal stem cells. This system may enable researchers and physicians to remotely regulate the timing, dose, and sequence of drug delivery on-demand, with a wide range of clinical applications ranging from tissue engineering to cancer treatment.
Citation/Publisher Attribution
Kennedy, S., Hu, J., Kearney, C., Skaat, H., Gu, L., Gentili, M., Vandenburgh, H., & Mooney, D. (2016). Sequential release of nanoparticle payloads from ultrasonically burstable capsules. Biomaterials, 75, 91-101. doi: 10.1016/j.biomaterials.2015.10.008
Available at: https://doi.org/10.1016/j.biomaterials.2015.10.008
Supplementary Data
Comment
Stephen Kennedy has a dual appointment with the Department of Electrical, Computer and Biomedical Engineering and the Department of Chemical Engineering.
Author Manuscript
This is a pre-publication author manuscript of the final, published article.
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