"STEADY-STATE AND DECAY CHARACTERISTICS OF PROTEIN TRYPTOPHAN FLUORESCE" by R. A. Dalterio, W. H. Nelson et al.
 

STEADY-STATE AND DECAY CHARACTERISTICS OF PROTEIN TRYPTOPHAN FLUORESCENCE FROM BACTERIA.

Document Type

Article

Date of Original Version

1-1-1986

Abstract

The intrinsic steady-state fluorescence and fluorescence decay of Staphylococcus epidermidis, Pseudomonas fluorescens, Enterobacter cloacae, Escherichia coli, and Bacillus subtilis have been observed. Each organism exhibits a strong maximum in its emission spectrum at 330-340 nm when excited at 290 nm. Iodide quenching and denaturization experiments with 8 M urea provide strong evidence for the assignment of the 330-340-nm fluorescence to protein tryptophan. Most importantly, the decay of this bacterial protein-tryptophan fluorescence has been described by two exponential functions in all cases.

Publication Title, e.g., Journal

Applied Spectroscopy

Volume

40

Issue

1

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