STEADY-STATE AND DECAY CHARACTERISTICS OF PROTEIN TRYPTOPHAN FLUORESCENCE FROM BACTERIA.
Document Type
Article
Date of Original Version
1-1-1986
Abstract
The intrinsic steady-state fluorescence and fluorescence decay of Staphylococcus epidermidis, Pseudomonas fluorescens, Enterobacter cloacae, Escherichia coli, and Bacillus subtilis have been observed. Each organism exhibits a strong maximum in its emission spectrum at 330-340 nm when excited at 290 nm. Iodide quenching and denaturization experiments with 8 M urea provide strong evidence for the assignment of the 330-340-nm fluorescence to protein tryptophan. Most importantly, the decay of this bacterial protein-tryptophan fluorescence has been described by two exponential functions in all cases.
Publication Title, e.g., Journal
Applied Spectroscopy
Volume
40
Issue
1
Citation/Publisher Attribution
Dalterio, R. A., W. H. Nelson, D. Britt, J. Sperry, D. Psaras, J. F. Tanguay, and S. L. Suib. "STEADY-STATE AND DECAY CHARACTERISTICS OF PROTEIN TRYPTOPHAN FLUORESCENCE FROM BACTERIA.." Applied Spectroscopy 40, 1 (1986). doi: 10.1366/0003702864815439.
