An in vitro assay to measure phagocytosis in striped bass hybrids (Morone saxatilis × Morone chrysops)
Document Type
Article
Date of Original Version
1-1-2000
Abstract
An in vitro phagocytosis assay was developed for hybrid striped bass (Morone saxatilis×Morone chrysops), using cells collected from the peritoneal cavity of this fish. The findings indicated that: (1) 10 days following a single intraperitoneal injection (1ml) of Freund's incomplete adjuvant (FIA) was an appropriate time for collecting suitable working concentrations (5·3± 4·0×107cells ml-1) of peritoneal phagocytes (83·7±1·5% macrophages) from these hybrids held at 23°C; (2) these cells phagocytosed latex beads (polystyrene microspheres 3·12μm in diameter) after 30min of in vitro incubation at room temperature (25±1°C). The phagocytic ability and phagocytic capacity in a washed adherent layer exposure system were 67·2±2·76% and 4·14±0·35 beads phagocyte-1, respectively. These results strongly suggest that a simple methodology, including baseline data serving as guidelines, is now available for conducting in vitro phagocytosis assays in this hybrid © 2000 Academic Press.
Publication Title, e.g., Journal
Fish and Shellfish Immunology
Volume
10
Issue
5
Citation/Publisher Attribution
Polonio, Roy E., Richard E. Wolke, Sharon A. Maclean, and Jay F. Sperry. "An in vitro assay to measure phagocytosis in striped bass hybrids (Morone saxatilis × Morone chrysops)." Fish and Shellfish Immunology 10, 5 (2000). doi: 10.1006/fsim.1999.0248.
