Intensities of E. coli nucleic acid Raman spectra excited selectively from whole cells with 251-nm light
Date of Original Version
Escherichia coli bacteria in the logarithmic growth phase have been investigated by IN resonance Raman spectroscopy. Bacterial whole-cell Raman spectra excited at 251 nm reflect nearly exclusively the nucleic acid composition even though a very large fraction of the bacterial mass is composed of protein. It has been demonstrated that if bacteria are grown under controlled (logarithmic growth) conditions, which give rise to organisms of known average biochemical composition, the intensities of E. coli Raman spectra can be explained quantitatively from the knowledge of component nucleic acid base resonance Raman cross sections.
Publication Title, e.g., Journal
Wu, Q., W. H. Nelson, S. Elliot, J. F. Sperry, M. Feld, R. Dasari, and R. Manoharan. "Intensities of E. coli nucleic acid Raman spectra excited selectively from whole cells with 251-nm light." Analytical Chemistry 72, 13 (2000). doi: 10.1021/ac990932p.