Selective detection of 1000 B. Anthracis spores within 15 minutes using a peptide functionalized SERS assay

Authors

Stuart Farquharson, Real-Time Analyzers, Inc.
Chetan Shende, Real-Time Analyzers, Inc.
Wayne Smith, Real-Time Analyzers, Inc.
Hermes Huang, Smiths Detection
Frank Inscore, Real-Time Analyzers, Inc.
Atanu Sengupta, Dr. Reddy's Laboratories Ltd. India
Jay Sperry, University of Rhode Island
Todd Sickler, Edgewood Chemical Biological Center
Amber Prugh, Edgewood Chemical Biological Center
Jason Guicheteau, Edgewood Chemical Biological Center

Document Type

Article

Date of Original Version

11-10-2014

Abstract

A surface-enhanced Raman spectroscopy (SERS) assay has been designed to detect Bacillus anthracis spores. The assay consists of silver nanoparticles embedded in a porous glass structure that have been functionalized with ATYPLPIR, a peptide developed to discriminately bind B. anthracis versus other species of Bacillus. Once bound, acetic acid was used to release the biomarker dipicolinic acid from the spores, which was detected by SERS through the addition of silver colloids. This SERS assay was used to selectively bind B. anthracis with a 100-fold selectivity versus B. cereus, and to detect B. anthracis Ames at concentrations of 1000 spores per mL within 15 minutes. The SERS assay measurements provide a basis for the development of systems that can detect spores collected from the air or from water supplies. © 2014 the Partner Organisations.

Publication Title, e.g., Journal

Analyst

Volume

139

Issue

24

Citation/Publisher Attribution

Farquharson, Stuart, Chetan Shende, Wayne Smith, Hermes Huang, Frank Inscore, Atanu Sengupta, Jay Sperry, Todd Sickler, Amber Prugh, and Jason Guicheteau. "Selective detection of 1000 B. Anthracis spores within 15 minutes using a peptide functionalized SERS assay." Analyst 139, 24 (2014). doi: 10.1039/c4an01163e.