Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus
Date of Original Version
Escherichia coli F-18 isolated from the feces of a healthy human is an excellent colonizer of the CD-1 mouse colon. In the present investigation, adhesion of E. coli F-18 to CD-1 mouse colonic mucus and bovine serum albumin (BSA), immobilized on polystyrene, was studied. Adhesion of E. coli F-18 to mucus was two- to sixfold greater than to either BSA or polystyrene. E. coli F-18 lipopolysaccharide specifically blocked adhesion of E. coli F-18 to mucus and mimicked adhesion of E. coli F-18 to mucus, BSA, and polystyrene. Purified capsule also blocked adhesion of E. coli F-18 to mucus, but this inhibition was found to be entirely nonspecific. The specific E. coli F-18 receptor in mucus appeared to be a glycoprotein, containing sugars normally found in mucins and having a maximum molecular weight of between 1.25x105 and 2.5x105.
Publication Title, e.g., Journal
Infection and Immunity
Cohen, P. S., J. C. Arruda, T. J. J. Williams, and D. C. Laux. "Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus." Infection and Immunity 48, 1 (1985): 139-145. doi: 10.1128/iai.48.1.139-145.1985.