Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus
Document Type
Article
Date of Original Version
1-1-1985
Abstract
Escherichia coli F-18 isolated from the feces of a healthy human is an excellent colonizer of the CD-1 mouse colon. In the present investigation, adhesion of E. coli F-18 to CD-1 mouse colonic mucus and bovine serum albumin (BSA), immobilized on polystyrene, was studied. Adhesion of E. coli F-18 to mucus was two- to sixfold greater than to either BSA or polystyrene. E. coli F-18 lipopolysaccharide specifically blocked adhesion of E. coli F-18 to mucus and mimicked adhesion of E. coli F-18 to mucus, BSA, and polystyrene. Purified capsule also blocked adhesion of E. coli F-18 to mucus, but this inhibition was found to be entirely nonspecific. The specific E. coli F-18 receptor in mucus appeared to be a glycoprotein, containing sugars normally found in mucins and having a maximum molecular weight of between 1.25x105 and 2.5x105.
Publication Title, e.g., Journal
Infection and Immunity
Volume
48
Issue
1
Citation/Publisher Attribution
Cohen, P. S., J. C. Arruda, T. J. J. Williams, and D. C. Laux. "Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus." Infection and Immunity 48, 1 (1985): 139-145. doi: 10.1128/iai.48.1.139-145.1985.