Stimulation of Escherichia coli F-18Col^- type-1 fimbriae synthesis by leuX

Authors

Joseph V. Newman, University of Rhode Island
Robert L. Burghoff, University of Rhode Island
Lars Pallesen, University of Rhode Island
Karen A. Krogfelt
Claus S. Kristensen, Technical University of Denmark
David C. Laux, University of Rhode Island
Paul S. Cohen, University of Rhode Island

Document Type

Article

Date of Original Version

10-1-1994

Abstract

Escherichia coli F-18, a normal human fecal isolate, is an excellent colonizer of the streptomycin-treated mouse large intestine. E. coli F-18Col-, a derivative of E. coli F-18 which no longer makes the E. coli F-18 colicin, colonizes the large intestine as well as E. coli F-18 when fed to mice alone but is eliminated when fed together with E. coli F-18. Recently we randomly cloned E. coli F-18 DNA into E. coli F-18Col- and let the mouse intestine select the best colonizer. In this way, we isolated a 6.5-kb E. coli F-18 DNA sequence that simultaneously stimulated synthesis of type 1 fimbriae and enhanced E. coli F-18Col- colonizing ability. In the present investigation we show that the gene responsible for stimulation of type 1 fimbriae synthesis appears to be leuX, which encodes a tRNA specific for the rare leucine codon UUG. Moreover, it appears that expression of leuX may be regulated by two proteins (22 kDa and 26 kDa) encoded by genes immediately adjacent to leuX. © 1994.

Publication Title, e.g., Journal

FEMS Microbiology Letters

Volume

122

Issue

3

Citation/Publisher Attribution

Newman, Joseph V., Robert L. Burghoff, Lars Pallesen, Karen A. Krogfelt, Claus S. Kristensen, David C. Laux, and Paul S. Cohen. "Stimulation of Escherichia coli F-18Col^- type-1 fimbriae synthesis by leuX." FEMS Microbiology Letters 122, 3 (1994): 281-287. doi: 10.1111/j.1574-6968.1994.tb07181.x.