Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery

Authors

Amir Nasrolahi Shirazi
Karissa L. Paquin, University of Rhode Island
Niall G. Howlett, University of Rhode IslandFollow
Dindyal Mandal
Keykavous Parang, University of Rhode IslandFollow

Document Type

Article

Date of Original Version

8-28-2014

Department

Cell & Molecular Biology

Abstract

Previously, we have reported the synthesis of a homochiral l-cyclic peptide [WR]₅ and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl₄ and the peptide generated peptide-capped gold nanoparticles. Here, [WR]₅ and [WR]₅-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]₅ and [WR]₅-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]₅ and [WR]₅-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.

Citation/Publisher Attribution

Shirazi AN, Paquin KL, Howlett NG, Mandal D, Parang K. (2014). "Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery." Molecules. 19(9): 13319-31.

Available at: http://dx.doi.org/10.3390/molecules190913319

Creative Commons License

This work is licensed under a Creative Commons Attribution 3.0 License.