Date of Original Version
Clostridium pasteurianum ATCC 6013 achieves high n-butanol production when glycerol is used as the sole carbon source. In this study, the homeoviscous membrane response of C. pasteurianum ATCC 6013 has been examined through n-butanol challenge experiments. Homeoviscous response is a critical aspect of n-butanol tolerance and has not been examined in detail for C. pasteurianum. Lipid membrane compositions were examined for glycerol fermentations with n-butanol production, and during cell growth in the absence of n-butanol production, using gas chromatography–mass spectrometry (GC–MS) and proton nuclear magnetic resonance (1H-NMR). Membrane stabilization due to homeoviscous response was further examined by surface pressure–area (π–A) analysis of membrane extract monolayers. C. pasteurianum was found to exert a homeoviscous response that was comprised of an increase lipid tail length and a decrease in the percentage of unsaturated fatty acids with increasing n-butanol challenge. This led to a more rigid or stable membrane that counteracted n-butanol fluidization. This is the first report on the changes in the membrane lipid composition during n-butanol production by C. pasteurianum ATCC 6013, which is a versatile microorganism that has the potential to be engineered as an industrial n-butanol producer using crude glycerol.
Venkataramanan, K. P., Kurniawan, Y., Boatman, J. J., Haynes, C. H., Taconi, K. A., Martin, L. M., Bothun, G. D., & Scholz, C. (2014). Homeoviscous response of Clostridium pasteurianum to butanol toxicity during glycerol fermentation. Journal of Biotechnology, 179, 8-14. doi: 10.1016/j.jbiotec.2014.03.017
Available at: https://doi.org/10.1016/j.jbiotec.2014.03.017