Over One Million DNA and Protein Events Through Ultra-Stable Chemically-Tuned Solid-State Nanopores
Document Type
Article
Date of Original Version
7-19-2023
Abstract
Stability, long lifetime, resilience against clogging, low noise, and low cost are five critical cornerstones of solid-state nanopore technology. Here, a fabrication protocol is described wherein >1 million events are obtained from a single solid-state nanopore with both DNA and protein at the highest available lowpass filter (LPF, 100 kHz) of the Axopatch 200B–the highest event count mentioned in literature. Moreover, a total of ≈8.1 million events are reported in this work encompassing the two analyte classes. With the 100 kHz LPF, the temporally attenuated population is negligible while with the more ubiquitous 10 kHz, ≈91% of the events are attenuated. With DNA experiments, the pores are operational for hours (typically >7 h) while the average pore growth is merely ≈0.16 ± 0.1 nm h−1. The current noise is exceptionally stable with traces typically showing <10 pA h−1 increase in noise. Furthermore, a real-time method to clean and revive pores clogged with analyte with the added benefit of minimal pore growth during cleaning (< 5% of the original diameter) is showcased. The enormity of the data collected herein presents a significant advancement to solid-state pore performance and will be useful for future ventures such as machine learning where large amounts of pristine data are a prerequisite.
Publication Title, e.g., Journal
Small
Volume
19
Issue
29
Citation/Publisher Attribution
Saharia, Jugal, Yapa Mudiyanselage Nuwan D. Bandara, Buddini I. Karawdeniya, Jason R. Dwyer, and Min Jun Kim. "Over One Million DNA and Protein Events Through Ultra-Stable Chemically-Tuned Solid-State Nanopores." Small 19, 29 (2023). doi: 10.1002/smll.202300198.