Lung cell membrane-coated nanoparticles capable of enhanced internalization and translocation in pulmonary epithelial cells
Document Type
Article
Date of Original Version
2-5-2022
Abstract
Cell membrane-coated nanoparticles (CMCNP), which involve coating a core nanoparticle (NP) with cell membranes, have been gaining attention due to their ability to mimic the properties of the cells, allowing for enhanced delivery and efficacy of therapeutics. Two CMCNP systems comprised of an acetalated dextran-based NP core loaded with curcumin (CUR) coated with cell membranes derived from pulmonary epithelial cells were developed. The NP were approximately 200 nm and their surface charges varied based on their coating, where CMCNP systems exhibited negative surface charge like natural cell membranes. The NP were smooth, spherical, and homogeneous with distinct coatings on their cores. Minimal in vitro toxicity was observed for the NP and controlled release of CUR was observed. The CMCNP internalized into and translocated across an in vitro pulmonary epithelial monolayer significantly more than the control NP. Blocking endocytosis pathways reduced the transcytosis of NP, indicating a relationship between endocytosis and transcytosis. These newly developed CMCNP have the potential to be used in pulmonary drug delivery applications to potentially enhance NP internalization and transport into and across the pulmonary epithelium.
Publication Title, e.g., Journal
International Journal of Pharmaceutics
Volume
613
Citation/Publisher Attribution
Jakaria, Md Golam, Parand Sorkhdini, Dongqin Yang, Yang Zhou, and Samantha A. Meenach. "Lung cell membrane-coated nanoparticles capable of enhanced internalization and translocation in pulmonary epithelial cells." International Journal of Pharmaceutics 613, (2022). doi: 10.1016/j.ijpharm.2021.121418.