Title

Multicomponent folate-targeted magnetoliposomes: design, characterization, and cellular uptake

Authors

Geoffrey D. Bothun, University of Rhode IslandFollow
Alline Lelis, University of Rhode Island
Yanjing Chen, University of Rhode Island
Kyle Scully, University of Rhode Island
Linnea E. Anderson, University of Rhode Island
Matthew A. Stoner, University of Rhode Island

Document Type

Article

Date of Original Version

2011

Abstract

Folate-targeted cationic magnetoliposomes (FTMLs) have been prepared with coencapsulated doxorubicin (DOX) and anionic superparamagnetic iron oxide (SPIO) nanoparticles (NPs) with 5 nm γ-Fe₂O₃ cores and 16 nm hydrodynamic diameters. NP encapsulation (89%) was confirmed by cryogenic transmission electron microscopy (TEM), and the presence of the oppositely charged NPs did not cause liposome aggregation. The FTMLs had an average diameter of 174 ± 53 nm and existed as unilamellar and cup-shaped liposomes, which was attributed to dissimilar lipid packing parameters and the presence of PEG-lipids. A 3-fold increase in DOX release was achieved over 2 hours when the encapsulated SPIO NPs were heated by an alternating current electromagnetic field operating at radio frequencies (RF). Results with human cervical cancer cells (HeLa), which have been shown to exhibit high folate receptor (FR) expression, confirmed FTML surface binding and cellular uptake. In contrast, no uptake was observed for lower FR-expressing human breast carcinoma cells (ZR-75-1).

[Refer to PDF for graphical abstract]

Citation/Publisher Attribution

Bothun, G. D., Lelis, A., Chen, Y., Scully, K., Anderson, L. E., & Stoner, M. A. (2011). Multicomponent folate-targeted magnetoliposomes: design, characterization, and cellular uptake. Nanomedicine: Nanotechnology, Biology and Medicine, 7(6), 797-805. doi: 10.1016/j.nano.2011.02.007

Available at: https://doi.org/10.1016/j.nano.2011.02.007

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Comment

Geoffrey D. Bothun and Yanjing Chen are from the Department of Chemical Engineering.

Kyle Scully and Matthew A. Stoner are from the Department of Biomedical and Pharmaceutical Sciences.

Alline Lelis has a dual appointment with Department of Chemical Engineering and Department of Biomedical and Pharmaceutical Sciences.