Chemical Engineering Faculty Publications

Low-dose chemotherapy of hepatocellular carcinoma through triggered-release from bilayer-decorated magnetoliposomes

Yanjing Chen, University of Rhode Island
Yuan Chen, University of Rhode Island
Da Xiao, University of Rhode Island
Arijit Bose, University of Rhode IslandFollow
Ruitang Deng, University of Rhode IslandFollow
Geoffrey D. Bothun, University of Rhode IslandFollow

Document Type

Article

Date of Original Version

2014

Abstract

Low-dose (LD) chemotherapy is a promising treatment strategy that may be improved by controlled delivery. Polyethylene glycol-stabilized bilayer-decorated magnetoliposomes (dMLs) have been designed as a stimuli-responsive LD chemotherapy drug delivery system and tested in vitro using Huh-7 hepatocellular carcinoma cell line. The dMLs contained hydrophobic superparamagnetic iron oxide nanoparticles within the lipid bilayer and doxorubicin hydrochloride (DOX, 2 μM) within the aqueous core. Structural analysis by cryogenic transmission electron microscopy and dynamic light scattering showed that the assemblies were approximately 120 nm in diameter. Furthermore, the samples consisted of a mixture of dMLs and bare liposomes (no nanoparticles), which provided dual burst and spontaneous DOX release profiles, respectively. Cell viability results show that the cytotoxicity of DOX-loaded dMLs was similar to that of bare dMLs (∼10%), which indicates that spontaneous DOX leakage had little cytotoxic effect. However, when subjected to a physiologically acceptable radiofrequency (RF) electromagnetic field, cell viability was reduced up to 40% after 8 h and significant cell death (>90%) was observed after 24 h. The therapeutic mechanism was intracellular RF-triggered DOX release from the dMLs and not intracellular hyperthermia due to nanoparticle heating via magnetic losses.

[Refer to PDF for graphical abstract]

Citation/Publisher Attribution

Chen, Y., Chen, Y., Xiao, D., Bose, A., Deng, R., & Bothun, G. (2014). Low-dose chemotherapy of hepatocellular carcinoma through triggered-release from bilayer-decorated magnetoliposomes. Colloids and Surfaces B: Biointerfaces, 116, 452-458. doi: 10.1016/j.colsurfb.2014.01.022

Available at: https://doi.org/10.1016/j.colsurfb.2014.01.022