In Vivo Colonization of the Mouse Large Intestine and In Vitro Penetration of Intestinal Mucus by an Avirulent Smooth Strain of Salmonella typhimurium and Its Lipopolysaccharide-Deficient Mutant

Authors

Joseph J. Nevola, University of Rhode Island
David C. Laux, University of Rhode IslandFollow
Paul S. Cohen, University of Rhode IslandFollow

Document Type

Article

Date of Original Version

1987

Department

Microbiology

Abstract

The relative abilities of an avirulent Salmonella typhimurium strain with wild-type lipopolysaccharide (LPS) character, SL5319, and a nearly isogenic LPS-deficient mutant, SL5325, to colonize the large intestines of streptomycin-treated CD-1 mice in vivo and to penetrate colonic mucus in vitro were studied. Previously it had been shown that, when fed simultaneously to streptomycin-treated mice (approximately 10¹⁰ CFU each), the S. typhimurium strain with wild-type LPS colonized at 10⁸ CFU/g of feces indefinitely, whereas the LPS-deficient mutant dropped within 3 days to a level of only 10⁴ CFU/g of feces. In the present investigation, when SL5325 was allowed to colonize for 8 days before feeding mice SL5319 or when it was fed to mice simultaneously with an Escherichia coli strain of human fecal origin (10¹⁰ CFU each), both strains colonized indefinitely at 10⁷ CFU/g of feces. Moreover, when the wild-type and LPS-deficient mutant strains were fed to mice simultaneously in low numbers (approximately 10⁵ CFU each) the strains survived equally well in the large intestines for 8 days, after which the LPS-deficient mutant was eliminated (less than 10² CFU/g of feces), whereas the wild-type colonized at a level of 10⁷ CFU/g of feces. In addition although both strains were able to adhere to mucus and epithelial cell preparations in vitro, the wild-type strain was shown to have greater motility and chemotactic activity on CD-1 mouse colonic mucus in vitro and to more rapidly penetrate and form a stable association with immobilized colonic mucosal components in vitro. Based on these data, we suggest that the ability of an S. typhimurium strain to colonize the streptomycin-treated mouse large intestine may, in part, depend on its ability to penetrate deeply into the mucus layer on the intestinal wall and subsequently, through growth, colonize the mucosa.

Citation/Publisher Attribution

Nevola, J. J., Laux, D. C., & Cohen, P. S. (1987). In Vivo Colonization of the Mouse Large Intestine and In Vitro Penetration of Intestinal Mucus by an Avirulent Smooth Strain of Salmonella typhimurium and Its Lipopolysaccharide-Deficient Mutant. Infection and Immunity, 55(12), 2884-2890. Retrieved from http://iai.asm.org/content/55/12/2884.long.

Available at: http://iai.asm.org/content/55/12/2884.long