A Functional cra Gene Is Required for Salmonella enterica Serovar Typhimurium Virulence in BALB/c Mice

Authors

James H. Allen, University of Rhode Island
Maryjane Utley, University of Rhode Island
Han van der Bosch
Piet Nuijten
Maarten Witvliet
Beth A. McCormick
Karen A. Krogfelt
Tine Rask Licht
Derek Brown
Michael Mauel, University of Rhode Island
Mary P. Leatham, University of Rhode Island
David C. Laux, University of Rhode IslandFollow
Paul S. Cohen, University of Rhode IslandFollow

Document Type

Article

Date of Original Version

2000

Department

Biochemistry, Microbiology and Molecular Genetics

Abstract

A minitransposon mutant of Salmonella enterica serovar Typhimurium SR-11, SR-11 Fad^-, is unable to utilize gluconeogenic substrates as carbon sources and is avirulent and immunogenic when administered perorally to BALB/c mice (M. J. Utley et al., FEMS Microbiol. Lett., 163:129–134, 1998). Here, evidence is presented that the mutation in SR-11 Fad^- that renders the strain avirulent is in the cra gene, which encodes the Cra protein, a regulator of central carbon metabolism.

Citation/Publisher Attribution

Allen, J. H., Utley, M., van den Bosch, H., Nuijten, P., Witvliet, M., McCormick, B. A., Krogfelt, K. A.,...Cohen, P. S. (2000). A Functional cra Gene Is Required for Salmonella enterica Serovar Typhimurium Virulence in BALB/c Mice. Infection and Immunity, 68(6), 3772-3775. doi: 10.1128/IAI.68.6.3772-3775.2000

Available at: http://dx.doi.org/10.1128/IAI.68.6.3772-3775.2000