Downregulating NF-κB signaling pathway with triterpenoids for attenuating inflammation:: In vitro and in vivo studies
Document Type
Article
Date of Original Version
8-1-2019
Abstract
Acanthopanax trifoliatus (L.) Merr., an edible medicinal plant from Southeast Asia, exerts a wide range of bioactivities, such as anti-inflammatory activity. However, the anti-inflammatory mechanisms of its action and active constituents remain unclear. Herein, the effects of two triterpenoids, namely impressic acid (IA) and acankoreanogenin A (AA), from A. trifoliatus in both in vitro and in vivo chronic inflammation models were investigated. The results indicated that AA and IA reduced lipopolysaccharide (LPS)-induced production of nitroxide significantly in murine macrophage RAW246.7 cells. In addition, AA and IA down-regulated the activation of NF-κB and decreased the release of inflammatory mediators (iNOS, COX-2, TNF-α, and IL-6) and tumorigenesis-associated factors (MMP-9 and VEGF) in RAW246.7 cells. Furthermore, in a tetradecanoylphorbolacetate (TPA)-treated mouse model, AA and IA could effectively attenuate mouse ear edema and pathological damage and reduced levels of cytokines including iNOS, COX-2, TNF-α, and IL-1β. Taken together, AA and IA, being of natural origin, are promising anti-inflammatory agents and may contribute to the overall anti-inflammatory effect of A. trifoliatus.
Publication Title, e.g., Journal
Food and Function
Volume
10
Issue
8
Citation/Publisher Attribution
Chen, Min, Ying Qin, Hang Ma, Xi Zheng, Renping Zhou, Shili Sun, Yiqi Huang, Qing Duan, Wenfeng Liu, Panpan Wu, Xuetao Xu, Zhaojun Sheng, Kun Zhang, and Dongli Li. "Downregulating NF-κB signaling pathway with triterpenoids for attenuating inflammation:: In vitro and in vivo studies." Food and Function 10, 8 (2019): 5080-5090. doi: 10.1039/c9fo00561g.