Synthesis and anti-HIV activities of symmetrical dicarboxylate esters of dinucleoside reverse transcriptase inhibitors

Authors

Hitesh K. Agarwal, University of Rhode Island
Karen W. Buckheit
Robert W. Buckheit Jr.
Keykavous Parang, University of Rhode IslandFollow

Document Type

Article

Date of Original Version

2012

Department

Biomedical and Pharmaceutical Sciences

Abstract

Three nucleoside analogues, 3′-fluoro-2′,3′-dideoxythymidine (FLT), 3′-azido-2′,3′-dideoxythymidine (AZT), and 2′,3′-dideoxy-3′-thiacytidine (3TC) were conjugated with three different dicarboxylic acids to afford the long chain dicarboxylate esters of nucleosides. In general, dinucleoside ester conjugates of FLT and 3TC with long chain dicarboxylic acids exhibited higher anti-HIV activity than their parent nucleosides. Dodecanoate and tetradecanoate dinucleoside ester derivatives of FLT were found to be the most potent compounds with EC₅₀ values of 0.8–1.0 nM and 3–4 nM against HIV-1_US/92/727 and HIV-1_IIIB cells, respectively. The anti-HIV activity of the 3TC conjugates containing long chain dicarboxylate diester (EC₅₀ = 3–60 nM) was improved by 1.5–66 fold when compared to 3TC (EC₅₀ = 90–200 nM). This study reveals that the symmetrical ester conjugation of dicarboxylic acids with a number of nucleosides results in conjugates with improved anti-HIV profile.

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Citation/Publisher Attribution

Agarwal, H. K., Buckheit, K. W., Buckheit, Jr., R. W., & Parang, K. (2012). Synthesis and anti-HIV activities of symmetrical dicarboxylate esters of dinucleoside reverse transcriptase inhibitors. Bioorganic & Medicinal Chemistry Letters, 22(17), 5451-5454. doi: 10.1016/j.bmcl.2012.07.037

Available at: https://doi.org/10.1016/j.bmcl.2012.07.037

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