Document Type
Article
Date of Original Version
2014
Department
Biomedical and Pharmaceutical Sciences
Abstract
The preventive effects of the American cranberry (Vaccinium macrocarpon) against urinary tract infections are supported by extensive studies which have primarily focused on its phenolic constituents. Herein, a phenolic-free carbohydrate fraction (designated cranf1b-F2) was purified from cranberry fruit using ion exchange and size exclusion chromatography. MALDI-TOF-MS analysis revealed that the cranf1b-F2 constituents are predominantly oligosaccharides possessing various degrees of polymerisation and further structural analysis (by GC–MS and NMR) revealed mainly xyloglucan and arabinan residues. In antimicrobial assays, cranf1b-F2 (at 1.25 mg/mL concentration) reduced biofilm production by the uropathogenic Escherichia coli CFT073 strain by over 50% but did not inhibit bacterial growth. Cranf1b-F2 (ranging from 0.625 to 10 mg/mL) also inhibited biofilm formation of the non-pathogenic E. coli MG1655 strain up to 60% in a concentration-dependent manner. These results suggest that cranberry oligosaccharides, in addition to its phenolic constituents, may play a role in its preventive effects against urinary tract infections.
Citation/Publisher Attribution
Sun, J., Marais, J. P.J., Khoo, C., LaPlante, K., Vejborg, R. M., Givskov, M., Tolker-Nielsen, T.,...Rowley, D. C. (2015). Cranberry (Vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli. Journal of Functional Foods, 17, 235-242. doi: 10.1016/j.jff.2015.05.016
Available at: https://doi.org/10.1016/j.jff.2015.05.016
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comment
Jiadong Sun and Navindra P. Seeram are from the Department of Biomedical and Pharmaceutical Sciences.
Kerry LaPlante is from the Department of Pharmacy Practice.
Author Manuscript
This is a pre-publication author manuscript of the final, published article.