Michael R. Kanost
Estela L. Arrese
Xiaolong Cao
Yun-Ru Chen
Sanjay Chellapilla
Marian R. Goldsmith, University of Rhode IslandFollow
Ewald Grosse-Wilde
David G. Heckel
Nicolae Herndon
Haobo Jiang
Alexie Papanicolaou
Jiaxin Qu
Jose L. Soulages
Heiko Vogel
James Walters
Robert M. Waterhouse
Seun-Joon Ahn
Francisca C. Almeida
Chunju An
Peshtewani Aqrawi
Anne Bretschneider
William B. Bryant
Sascha Bucks
Hsu Chao
Germain Chevignon
Jayne M. Christen
David F. Clarke
Neal T. Dittmer
Laura C.F. Ferguson
Spyridoula Garavelou
Karl H.J. Gordon
Ramesh T. Gunaratna
Yi Han
Frank Hauser
Yan He
Hanna Heidel-Fischer
Ariana Hirsh
Yingxia Hu
Hangbo Jiang
Divya Kalra
Christian Klinner
Christopher König
Christie Kovar
Ashley R. Kroll
Suyong S. Kuwar
Sandy L. Lee
Rüdiger Lehman
Kai Li
Zhaofei Li
Hanquan Liang
Shanna Lovelace
Zhiqiang Lu
Jennifer H. Mansfield
Kyle J. McCulloch
Tittu Matthew
Brian Morton
Donna M. Muzny
David Neumann
Fiona Ongeri
Yannick Pauchet
Ling-Ling Pu
Ioannis Pyrousis
Xiang-Jun Rao
Amanda Redding
Charles Roesel
Alejandro Sanchez-Gracia
Sarah Schaack
Aditi Shukla
Guillame Tetreau
Yang Wang
Guang-Hua Xiong
Walther Traut
Tom K. Walsh
Kim C. Worley
Di Wu
Wenbi Wu
Yuang-Qing Wu
Xiufeng Zhang
Zhen Zou
Hannah Zucker
Adriana D. Briscoe
Thorsten Burmester
Rollie J. Clem
René Feyereisen
Cornelius J.P. Grimmelikhuijzen
Stavros J. Hamodrakes
Bill S. Hansson
Elisabeth Huguet
Lar S. Jermiin
Que Lan
Herman K. Lehman
Marce Lorenzen
Hans Merzendorfer
Ioannis Michalopoulos
David B. Morton
Subbaratnam Muthukrishnan
John G. Oakeshott
Will Palmer
Yoonseong Park
A. Lorena Passarelli
Julio Rozas
Lawrence M. Schwartz
Wendy Smith
Agnes Southgate
Andreas Vilcinskas
Richard Vogt
Ping Wang
John Werren
Xiao-Qiang Yu
Jing-Jiang Zhou
Susan J. Brown
Steven E. Scherer
Stephen Richards
Gary W. Blissard

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Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.