Knocking out the wall: protocols for gene targeting in Physcomitrella patens.
Document Type
Article
Date of Original Version
6-3-2011
Abstract
The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the P. patens genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. The protocols described in this chapter provide methods for targeted gene knockout in P. patens, from constructing vectors and maintaining cultures to transforming protoplasts and analyzing the genotypes and phenotypes of the resulting transformed lines.
Publication Title, e.g., Journal
Methods in molecular biology (Clifton, N.J.)
Volume
715
Citation/Publisher Attribution
Roberts, Alison W., Christos S. Dimos, Michael J. Budziszek, Chessa A. Goss, and Virginia Lai. "Knocking out the wall: protocols for gene targeting in Physcomitrella patens.." Methods in molecular biology (Clifton, N.J.) 715, (2011): 273-290. doi: 10.1007/978-1-61779-008-9_19.