Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens
Date of Original Version
The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the P. patens genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. The protocols described in this chapter provide methods for targeted gene knockout in P. patens, from constructing vectors and maintaining cultures to transforming protoplasts and analysing the genotypes and phenotypes of the resulting transformed lines.
Publication Title, e.g., Journal
Methods in Molecular Biology
Roberts, Alison W., Christos S. Dimos, Michael J. Budziszek, Chessa A. Goss, Virginia Lai, and Arielle M. Chaves. "Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens." Methods in Molecular Biology 2149, (2020): 125-144. doi: 10.1007/978-1-0716-0621-6_8.