Targeted mutagenesis in bombyx mori using talens
Date of Original Version
Bombyx mori is a valuable model organism of high economic importance. Its genome sequence is available, as well as basic genetic and molecular genetic tools and markers. The introduction of genome editing methods based on engineered nucleases enables precise manipulations with genomic DNA, including targeted DNA deletions, insertions, or replacements in the genome allowing gene analysis and various applications. We describe here the use of TALENs which have a simple modular design of their DNA-binding domains, are easy to prepare and proved to be efficient in targeting of a wide range of cleavage sites. Our procedure often allows the production of individuals carrying homozygous mutations as early as in the G 1 generation
Methods in Molecular Biology
Takasu, Yoko, Toshiki Tamura, Marian Goldsmith, and Michal Zurovec. "Targeted mutagenesis in bombyx mori using talens." Methods in Molecular Biology 1338, (2016): 127-142. doi:10.1007/978-1-4939-2932-0_11.