Document Type
Article
Date of Original Version
2007
Department
Biological Sciences
Abstract
To facilitate the handling of small invertebrate embryos during whole-mount in situ hybridization, a method of solution exchange using laboratory mini-columns was developed. This protocol speeds time-consuming aspiration of buffers, and avoids accidental loss of the embryos, by gently pushing solutions through the column using air pressure from a syringe after each incubation. The next buffer is then added using a pipettor. Embryos are retained on a filter within the column. As many columns as desired may be processed in parallel for different probes or stages.
Citation/Publisher Attribution
Irvine S.Q. "Whole-mount in situ hybridization of small invertebrate embryos using laboratory mini-columns," BioTechniques 43:764-768 (2007).
Terms of Use
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