Date of Award
1981
Degree Type
Thesis
Degree Name
Master of Science in Chemical Engineering (MSChE)
Department
Chemical Engineering
First Advisor
Stanley M. Barnett
Abstract
The feasibility of using potato wastes as substrate for or single cell protein (SCP) and extracellular enzyme production by Pleurotus ostreatus was· studied in submerged culture.
Cell mass yield and enzyme production of P. ostreatus we re studied as a function of (1) substrate concentration, (2) source of nitrogen, (3) temperature, (4) pH and (5) the addition of sodium bisulfite on the medium. The fermentation process was carried out to batch cultures in 250 ml flasks . Protein content on a dry cell mass basis, alphaamylase activity and reducing sugar content in the broth were determined in all the growth steps of P. ostreatus.
Ammonium sulfate was found to be the better nitrogen source than either urea or ammonium nitrate for cell mass yield, protein content and alpha-amylase production.
Two additional batch experiments were carried out in a 5-liter fermenter as scale up of the optimum growth conditions determined in 250 ml flask culture. The results were close to those obtained in 250 ml flask.
Recommended Citation
Arenas Santiago, Adilades A., "Potato Waste as a Substrate for Single Cell Protein and Enzyme Production" (1981). Open Access Master's Theses. Paper 1388.
https://digitalcommons.uri.edu/theses/1388
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