Date of Award


Degree Type


Degree Name

Master of Science in Oceanography



First Advisor

Melissa Omand


Achieving depth-resolved particle-specific data in sparse, highly variable oceanic environments persists as a methodological challenge. Holography has emerged as a tool for in-situ imaging of microscopic organisms and other particles in the marine environment; appealing because of the relatively larger volume and simple optical configuration compared to other imaging systems. The digital in-line holographic microscope (DIHM) used in this study samples ~100× larger volumes than comparable objective lens-based systems, and is deployable on CTD-rosette, flow-through, and autonomous systems. However, it’s quantitative capabilities have so far, remained uncertain. Here, the quantitative skill of the DIHM to evaluate size and concentration of marine particles ranging from 5 to 1000 μm in diameter is assessed. Over one million particles are analyzed using a custom image processing pipeline, which allows a precise characterization of the three-dimensional volume sampled. These results are compared with the FlowCam, the Imaging FlowCytobot and traditional microscope counts through laboratory and field-based inter-calibration experiments. Based on this analysis, recommendations for achieving quantitive size and concentration measurements from the DIHM are suggested.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.



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