Title

Immunological characterization of bovine lysyl oxidase

Document Type

Article

Date of Original Version

1-1-1985

Abstract

1. 1. Antibodies to homogeneously purified bovine aortic lysyl oxidase were prepared in chickens. 2. 2. The chicken anti-lysyl oxidase antiserum effectively inhibited bovine aortic lysyl oxidase activity. Non-immune antiserum from chickens, goats and humans was found to enhance bovine aortic lysyl oxidase activity, while non-immune rabbit serum inhibited enzyme activity. 3. 3. A competitive ELISA was developed to monitor immunoreactive lysyl oxidase during purification. 4. 4. Chromatography of bovine lysyl oxidase on Sephacryl S-200, the final step in purification, revealed two peaks of immunoreactive lysyl oxidase. The large molecular weight peak appears to contain inactive multimeric forms of the enzyme. © 1985.

Publication Title

Comparative Biochemistry and Physiology -- Part B: Biochemistry and

Volume

81

Issue

4

Share

COinS