Hydroxylation of β-Carotene on Micro-Cel C
Date of Original Version
β-Carotene underwent substantial hydroxylation to isocryptoxanthin (4-hydroxy-β-carotene) when brought in contact with Micro-Cel C, an adsorbent long presuined to be inert. The reaction was rapid, the maximum accumulation rate of isocryptoxanthin occurring within the first 15 min. The extent of hydroxylation depended directly on the amount of Micro-Cel C; the percent yield of 1 mg of β-carotene ranged from 23% with 10 g of Micro-Cel C to 65% with 30 g of Micro-Cel C. Isozeaxanthin (4,4′-dihydroxy-β-carotene) was also formed but at markedly lower amounts. Very small quantities of mutatochrome, echinenone, 4-hydroxy-5′,8′-epoxy-β-carotene, and dehydro-β-carotene were also detected. The hydroxylating property of Micro-Cel C was not observed in other adsorbents such as silica gel, kieselguhr, Celite, alumina, HyfloSupercel, and MgO. © 1976, American Chemical Society. All rights reserved.
Journal of Agricultural and Food Chemistry
Rodriguez, Delia B., Kenneth L. Simpson, Tung Ching Lee, Clinton O. Chichester, Yohito Tanaka, and Teruhisa Katayama. "Hydroxylation of β-Carotene on Micro-Cel C." Journal of Agricultural and Food Chemistry 24, 4 (1976): 819-822. doi:10.1021/jf60206a052.