Response of rats to amino acid supplementation of brown egg albumin
Date of Original Version
The effect of amino acid supplementation on the relative nutritive value (RNV) of brown egg albumin (protein:glucose 3:2; 70% RH; 37°C; 30 days) was studied in weanling male albino rats. Impaired availability of essential amino acids was found in the Maillard protein before and after dialysis by in vitro enzyme digestion and amino acid assay. Rats were fed 3, 6 and 10% protein for 21 days, and the following RNV with respect to untreated egg albumin (100) were obtained: 7.2 for dialyzed Maillard protein; 3.9 for the undialyzed brown product; 85.7 for the dialyzed Maillard protein as supplemented with all the essential amino acids that were microbiologically unavailable, and 83.9 for the undialyzed brown product supplemented as above. The protein efficiency ratio (PER) evaluation failed to detect the difference between the supplemented Maillard and the untreated proteins. From the portal vein amino acid patterns of rats fed a single meal of Maillard egg albumin it was apparent that: the supply of most amino acids was decreased and lysine, arginine and isoleucine were the 3 least available amino acids after browning. Analysis of the total fecal nitrogen and amino acids corroborated the unavailability of most essential amino acids from brown egg albumin.
Journal of Nutrition
Sgarbieri, V. C., J. Amaya, M. Tanaka, and C. O. Chichester. "Response of rats to amino acid supplementation of brown egg albumin." Journal of Nutrition 103, 12 (1973): 1731-1738. doi:10.1093/jn/103.12.1731.