Date of Award

2017

Degree Type

Capstone Project

First Advisor

Bahram Nassersharif

Abstract

This final design report highlights the developments of the Lab on Paper over the past year. The goal is to redesign the Lab on Paper to be more easily manufactured and eliminate the hook effect which causes false negatives in lateral flow assays. Sponsor Labonachip, LLC desires to bring Lab on Paper to market and the easiest way to do so is to adapt it to existing manufacturing lines. By eliminating the hook effect, Lab on Paper will be more accurate and reliable than current lateral flow assays.

The Lab on Paper aims to become a universal diagnostic instrument that can be used by the average consumer with no training involved. What sets it apart from existing lateral flow technologies, such as pregnancy tests, is that it can utilize several reagents in a single test that can be sequentially delivered to the test area. This allows for more accurate and more sensitive tests as well as being able to measure the analyte concentration of a sample quantitatively through a color change. In addition, Lab on Paper has the potential to produce enzyme-linked immunosorbent assays which are typically done in a medical diagnostics lab and has not been implemented on a lateral flow test.

A solution to address this problem is to adapt the wax version of the Lab on Paper to a strip based form that resembles a traditional lateral flow assay. This will make the Lab on Paper more attractive to prospective manufacturers which will thereby introduce Lab on Paper to the commercial market. Another part of this redesign is to rearrange the chemistry involved in the test to ensure that marker antibodies bind to an analyte-capture antibody substrate in order to increase binding percentage.

Comments

Sponsor: Labonachip, LLC

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