Analysis of green tea constituents by HPLC-FTIR
Date of Original Version
HPLC-FTIR was investigated for the qualitative determination of catechins and methyl xanthines present in green tea extracts. A reversed phase separation of the green tea components was performed on a C-18 column equilibrated at 30°C using an isocratic mobile phase of acetonitrile: 0.1% formic acid (15:85), prior to introduction to the deposition interface linked to the FTIR detector. The solvent was evaporated at 130°C and spectra were collected every six seconds during the run. Six catechins, (+)-catechin, gallocatechin, (-)-epicatechin, (-)-epigallocatechin and (-)-epigallocatechin gallate, and (-)-epicatechin gallate, as well as two methyl xanthines, caffeine and theobromine, were separated and positively identified in a sample of Chinese green tea. The catechins and methyl xanthines were matched to the respective standards by both retention time and the measured infrared spectrum.
Publication Title, e.g., Journal
Journal of Liquid Chromatography and Related Technologies
Robb, Christina S., Susan E. Geldart, John A. Seelenbinder, and Phyllis R. Brown. "Analysis of green tea constituents by HPLC-FTIR." Journal of Liquid Chromatography and Related Technologies 25, 5 (2002). doi: 10.1081/JLC-120003036.