Title

Synthesis of acid protease from Mucor miehei: integration of production and recovery

Document Type

Article

Date of Original Version

1-1-1995

Abstract

The production of acid protease from Mucor Miehei was repressed by amino acid additions (3·5-5·0 g/litre) from hydrolysed casein. The integration of the batch bioprocess with an ultrafiltration unit proved effective in depressing enzyme production. A hollow fibre ultrafilter with a 5000 MW cutoff was used to reduce the concentration of amino acids present in the medium after enzyme concentration began to decline. Volume concentrating ratio (VCR) values of 2 and 3 were used for the experiments. Increases as high as 25% in enzyme concentration were achieved. © 1995.

Publication Title

Process Biochemistry

Volume

30

Issue

8

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