Synthesis of acid protease from Mucor miehei: integration of production and recovery
Date of Original Version
The production of acid protease from Mucor Miehei was repressed by amino acid additions (3·5-5·0 g/litre) from hydrolysed casein. The integration of the batch bioprocess with an ultrafiltration unit proved effective in depressing enzyme production. A hollow fibre ultrafilter with a 5000 MW cutoff was used to reduce the concentration of amino acids present in the medium after enzyme concentration began to decline. Volume concentrating ratio (VCR) values of 2 and 3 were used for the experiments. Increases as high as 25% in enzyme concentration were achieved. © 1995.
Escobar, Jose, and Stanley M. Barnett. "Synthesis of acid protease from Mucor miehei: integration of production and recovery." Process Biochemistry 30, 8 (1995): 695-700. doi:10.1016/0032-9592(95)00005-4.