Date of Original Version
Biochemistry, Microbiology and Molecular Genetics
Clostridium difficile ATCC 43255 fermented less than 10% of the mannitol in a medium at pH 7; however, when the initial pH of the medium was adjusted to 8.5 or 9, about 80% of the mannitol was fermented. Cell extracts of C. difficile phosphorylated mannitol with phosphoenolpyruvate, not ATP, indicating a phosphoenolpyruvate phosphotransferase system transport phosphorylation of mannitol. The phosphorylation product was dehydrogenated by D-mannitol-1-phosphate:NAD oxidoreductase. Growth at an initial pH of 8.5 yielded cytotoxin titers of 107 to 108 in Trypticase-yeast extract-mannitol medium, with a titer of 108 as early as 13 h.
Kazamias, M. T. & Sperry, J. F. (1995). Enhanced Fermentation of Mannitol and Release of Cytotoxin by Clostridium difficile in Alkaline Culture Media. Applied and Environmental Microbiology, 61(6), 2425-2427. Retrieved from https://aem.asm.org/content/61/6/2425.
Available at: https://aem.asm.org/content/61/6/2425