Title
Sequence Dependent Repair of 1,N6-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
Document Type
Article
Date of Original Version
2021
Department
Biomedical and Pharmaceutical Sciences
Abstract
Mutation patterns of DNA adducts, such as mutational spectra and signatures, are useful tools for diagnostic and prognostic purposes. Mutational spectra of carcinogens derive from three sources: adduct formation, replication bypass, and repair. Here, we consider the repair aspect of 1,N6-ethenoadenine (εA) by the 2-oxoglutarate/Fe(II)-dependent AlkB family enzymes. Specifically, we investigated εA repair across 16 possible sequence contexts (5′/3′ flanking base to εA varied as G/A/T/C). The results revealed that repair efficiency is altered according to sequence, enzyme, and strand context (ss- versus ds-DNA). The methods can be used to study other aspects of mutational spectra or other pathways of repair.
Publication Title, e.g., Journal
Molecules
Volume
26
Issue
17
Citation/Publisher Attribution
Qi, R., Bian, K., Chen, X., Tang, Q., Zhou, X., & Li, D. (2021). Sequence Dependent Repair of 1,N6-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB. Molecules, 26(17), 5285. https://doi.org/10.3390/molecules26175285
Available at: https://doi.org/10.3390/molecules26175285
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.