Date of Award

2014

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Civil and Environmental Engineering

First Advisor

Vinka Oyanedel-Craver

Abstract

Microarray technology is a high-throughput technique that allows a researcher to reduce the amount of reagents for each experiment as well as conduct multiple experiments with varying conditions simultaneously with the capability of analyzing this data quickly. Such microarray techniques have been applied in different fields such as environmental, pharmaceutical, microbiology, and biomedical among others (Kricka 2001). Chapter 1 provides an introduction to microarray technology with a description of three different techniques: Phenotypic Microarray, DNA Microarray and Protein Microarray, used to study different aspects of microorganisms. Chapter 2 presents the first manuscript, “A novel application of microarray technology to respiration study of Escherichia coli”, with a focus on the development of a respirometric protocol to monitor the respiration of Escherichia coli over time. Chapter 3 presents the second manuscript, “Effect of Dysprosium oxide nanoparticles on Escherichia coli”, with a focus on the application of the developed respirometric methodology in Chapter 2 to the toxicity effect of dysprosium oxide nanoparticles on Escherichia coli. Chapter 4 presents the third manuscript, “Anaerobic growth of Acidithiobacillus ferrooxidans using microarray technology”, with a focus on the development of an anaerobic growth methodology for the reduction of iron to be used in future nanotoxicity studies.

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