Date of Award

2014

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Mechanical Engineering and Applied Mechanics

First Advisor

Mohammad Faghri

Abstract

This research has the focus on developing a paper-based device capable of sequentially flowing three separate fluids. Different assembly methods are investigated and compared to develop a more streamlined approach for the prototyping and testing of biological reagents. A faster method of fabrication is developed followed by an investigation of the potentials to detect the presence of Inter α Inhibitor Protein (a biomarker associated with sepsis). This is primarily achieved by applying the techniques of an Enzyme Linked Immunosorbent Assay (ELISA) onto a paper based platform. The research presented shows the steady progression toward and optimization of a lateral flow device capable of achieving the above and eventually presenting the capability of a competitive ELISA to detect IαIP. This platform allows for the possibility to generate results within a fraction of an hour and furthermore with smaller fluid volumes as compared to current bench top tests, such as ELISA which is typically conducted in 96 well plates.

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