Date of Award
Master of Science in Pharmacology and Toxicology
Pharmacology and Toxicology
Robert L. Rodgers
Diabetes mellitus is associated with a reduced responsiveness to catecholamines. The reduced responsiveness may be attributable to a reduction in beta-adrenoceptor sensitivity to catecholamines. Radioligand binding studies demonstrate that chemically-induced diabetes reduces beta-adrenoceptor number without altering betaadrenoceptor drug binding affinities. The present study re-examines the effect of chronic (10 weeks) streptozotocin-induced diabetes in the rat on beta-adrenoceptor drug binding affinity, using pharmacological techniques, to test the results of the binding studies. The study employed two different methods: partial irreversible receptor blockade and the use of a partial agonist, to determine betaadrenoceptor agonist binding affinity and the method of competitive antagonism to determine beta-adrenoceptor antagonist binding affinity. Diabetes produced no significant differences in the dissociation constants (l/affinity) for isoproterenol or for metaproterenol and no significant differences in the pAz values for timolol maleate which correlates to antagonist binding affinity. Therefore, the study confirms the results of radioligand binding studies by using intact tissue that diabetes does not alter beta-adrenoceptor drug binding affinity in cardiac tissue.
McKenna, Edward James, "BINDING AFFINITY OF BETA-ADRENOCEPTORS IN ATRIA OF STREPTOZOTOCIN-INDUCED DIABETIC AND NORMAL RATS" (1983). Open Access Master's Theses. Paper 207.